Mitigation of arsenic poisoning induced oxidative stress and genotoxicity by Ocimum gratissimum L

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F24%3A00601097" target="_blank" >RIV/61389030:_____/24:00601097 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15310/24:73628379

Result on the web

<a href="https://doi.org/10.1016/j.toxicon.2024.107603" target="_blank" >https://doi.org/10.1016/j.toxicon.2024.107603</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.toxicon.2024.107603" target="_blank" >10.1016/j.toxicon.2024.107603</a>

Result language

angličtina

Original language name

Mitigation of arsenic poisoning induced oxidative stress and genotoxicity by Ocimum gratissimum L

Original language description

Arsenic toxicity is a major problem across the world due to geogenic activity and has been supposed to generate free radicals and genotoxicity among the arsenic-poisoned population. There is a need to find suitable free radical quenching compounds for the arsenic-induced free radical-affected population. In the present study, Na3AsO3- induced oxidative stress and genotoxicity were evaluated in Oryctolagus cuniculus L, and quenching competency of Ocimum species was examined by applying enzymatic and non-enzymatic in vitro tests, comet assay, and Random Amplified Polymorphic Deoxyribonucleic acid Polymerase Chain Reaction (RAPD-PCR) methods. In the present study, oxidative damage due to Na3AsO3 intoxication in O. cuniculus L has been confirmed followed by substantive genotoxicity, and in a further study, it has also been reported that the extract of O. gratissimum L lowers the oxidative stress in experimental animals confirmed by a decrease in Malondialdehyde (MDA) 4.78 +/- 0.05 (nmol/mg protein), and an increase in Glutathione (GSH) 2.87 +/- 0.50 (mu moles/mg proteins), Superoxide Dismutase (SOD) 1.78 +/- 0.03(Units/mg protein), Catalase (CAT) 2.72 +/- 0.02 (mu moles of H2O2 consumed/min/mg proteins) and Glutathione peroxidase (GPX) 7.43 +/- 0.01 (mu g of glutathione utilized/min/mg protein). A positive impact of extract of O. gratissimum L on protection of genotoxicity has been also confirmed by Random Amplified Polymorphic DNA (RAPD) based reduction in polymorphic bands of Deoxyribonucleic acid (DNA) from 6.5 to 3.16 and comet assay-based increase in head DNA % (87.86 +/- 1.58), tail moment (1.07 +/- 0.27) and decrease in tail DNA % (12.13 +/- 1.58) & tail length (8.2 +/- 1.46) at 5% P in lymphocytes. A significant level reduction in free radicals and reduction in DNA polymorphism has proved the competency of test material for the development of suitable antidotes against arsenicosis.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30205 - Hematology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Toxicon

ISSN

0041-0101

e-ISSN

1879-3150

Volume of the periodical

238

Issue of the periodical within the volume

FEB 1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

9

Pages from-to

107603

UT code for WoS article

001157660100001

EID of the result in the Scopus database

2-s2.0-85182432567