Structural insights into brassinosteroid export mediated by the Arabidopsis ABC transporter ABCB1

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00618266" target="_blank" >RIV/61389030:_____/25:00618266 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15310/25:73634904

Result on the web

<a href="https://doi.org/10.1016/j.xplc.2024.101181" target="_blank" >https://doi.org/10.1016/j.xplc.2024.101181</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.xplc.2024.101181" target="_blank" >10.1016/j.xplc.2024.101181</a>

Result language

angličtina

Original language name

Structural insights into brassinosteroid export mediated by the Arabidopsis ABC transporter ABCB1

Original language description

Brassinosteroids (BRs) are steroidal phytohormones indispensable for plant growth, development, and responses to environmental stresses. The export of bioactive BRs to the apoplast is essential for BR signaling initiation, which requires binding of a BR molecule to the extracellular domains of the plasma membrane- localized receptor complex. We have previously shown that the Arabidopsis thaliana ATP-binding cassette (ABC) transporter ABCB19 functions as a BR exporter and, together with its close homolog ABCB1, positively regulates BR signaling. Here, we demonstrate that ABCB1 is another BR transporter. The ATP hydrolysis activity of ABCB1 can be stimulated by bioactive BRs, and its transport activity was confirmed in proteoliposomes and protoplasts. Structures of ABCB1 were determined in substrate-unbound (apo), brassinolide (BL)-bound, and ATP plus BL-bound states. In the BL-bound structure, BL is bound to the hydrophobic cavity formed by the transmembrane domain and triggers local conformational changes. Together, our data provide additional insights into ABC transporter-mediated BR export.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10611 - Plant sciences, botany

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Communications

ISSN

2590-3462

e-ISSN

2590-3462

Volume of the periodical

6

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

13

Pages from-to

101181

UT code for WoS article

001416757300001

EID of the result in the Scopus database

2-s2.0-85212316925