Phytochemical, LC-MS Studies, and Assessment of Antioxidant, Anti-inflammatory, Hypoglycemic, Anti-Rheumatoid-arthritis Potential with Acmella paniculata: In-Silico and In-Vitro Appraisal
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389030%3A_____%2F25%3A00635922" target="_blank" >RIV/61389030:_____/25:00635922 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1002/slct.202405189" target="_blank" >https://doi.org/10.1002/slct.202405189</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/slct.202405189" target="_blank" >10.1002/slct.202405189</a>
Alternative languages
Result language
angličtina
Original language name
Phytochemical, LC-MS Studies, and Assessment of Antioxidant, Anti-inflammatory, Hypoglycemic, Anti-Rheumatoid-arthritis Potential with Acmella paniculata: In-Silico and In-Vitro Appraisal
Original language description
Acmella paniculata (AP), an ethnomedicinal plant, is valued for the management of pain, inflammation, arthritis, infection, diabetes and as tribal cuisine The study focused on validating AP against inflammatory pain, rheumatoid arthritis (RA), and hyperglycemia. Hydro-ethanolic extract of AP flower (HEFeAP) was prepared via maceration, followed by ultrasonication. Physicochemical characterization was done using Thin Layer Chromatography (TLC), Liquid Chromatography-Mass Spectrometry (LC-MS), Ultra-violet Spectroscopy (UV), and Fourier Transform Infrared Spectroscopy (FTIR). Major phytochemicals were assessed against inflammatory mediators using molecular docking and Molecular Dynamics (MD) simulations. The antioxidant potential of HEFeAP was assessed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay (31.22 +/- 0.53 mu g mL-1) and hydrogen peroxide (H2O2) scavenging assay (40.36 +/- 0.47 mu g mL-1). The anti-inflammatory activity, protective role against RA, and anti-hyperglycaemic activities were evaluated via egg-albumin denaturation, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay against human-fibroblast cells and alpha-amylase inhibition, showing IC50 of 27.61, 65.75, and 85.43 mu g mL-1, respectively. Tumor Necrosis Factor-alpha (TNF-alpha), and nitric-oxide (NO) levels were significantly downregulated in LPS-stimulated PBMC-derived macrophages (IC50 = 152 and 164.56 mu g mL-1). Phytochemical analysis revealed coumarins, flavonoids, polyphenols, steroids, and local-anaesthetic alkylamides. Active phytomolecules in HEFeAP exhibited strong and stable binding with inflammatory biomarkers. These findings suggest HEFeAP can be used as a potential candidate for inflammatory pain, RA, and diabetes.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10609 - Biochemical research methods
Result continuities
Project
<a href="/en/project/GA23-05389S" target="_blank" >GA23-05389S: Novel CB2 and BChE modulators against Parkinson's disease and related pathologies</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ChemistrySelect
ISSN
2365-6549
e-ISSN
2365-6549
Volume of the periodical
10
Issue of the periodical within the volume
17
Country of publishing house
US - UNITED STATES
Number of pages
23
Pages from-to
e202405189
UT code for WoS article
001479588400001
EID of the result in the Scopus database
2-s2.0-105004211704