Liquid chromatography-tandem mass spectrometry method for simultaneous determination of cyclosporine A and its three metabolites AM1, AM9 and AM4N in whole blood and isolated lymphocytes in renal transplant patients
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17110%2F10%3AA110107E" target="_blank" >RIV/61988987:17110/10:A110107E - isvavai.cz</a>
Alternative codes found
RIV/00843989:_____/10:00101263
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Liquid chromatography-tandem mass spectrometry method for simultaneous determination of cyclosporine A and its three metabolites AM1, AM9 and AM4N in whole blood and isolated lymphocytes in renal transplant patients
Original language description
A LC-MS/MS method was developed and validated for the determination of cyclosporine A (CsA) and its three phase l metabolites AM1, AM9, and AM4N in whole blood and lymphocates isolated on the Histopaque gradient. 200 micro of whole blood was preciitatedwith 10 mol/L zinc sulfute in acetonitrile/methanol (40:60, v/v) and lymphocatesisolated from 1,5 mL blood were wstracted with acetonitrile/methanol (40:60, v/v). The analytes and internal standard cyclosporine D were separated on RP column BEH C18, 2,1x50 mm, 1,7 micro using gradient LC-MS/MS analysis in positive electrospray mode. Time of analysis was 5 min. Linearity in blood was 5-2000 micro/L for CsA, AM1, and AM9; 2-500 micro/L for AM4N; and 2-500 micro/L for all substances in lymphocytes. Coefficient of variations was 1,8-9,8% and recovery was 92,0-110,0 %.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FR - Pharmacology and apothecary chemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/1A8655" target="_blank" >1A8655: New Possibilities of TDM of Cyclosporine A and its Metabolites after kidney transplantation</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
J SEP SCI
ISSN
1615-9306
e-ISSN
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Volume of the periodical
33
Issue of the periodical within the volume
18.5. 2010
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
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UT code for WoS article
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EID of the result in the Scopus database
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