Activity of aldehyde dehydrogenase in B-cell and plasma cell subsets of monoclonal gammopathy patients and healthy donors
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17110%2F17%3AA1801R36" target="_blank" >RIV/61988987:17110/17:A1801R36 - isvavai.cz</a>
Alternative codes found
RIV/65269705:_____/17:00066856 RIV/00843989:_____/17:E0105801
Result on the web
<a href="http://dx.doi.org/10.1111/ejh.12779" target="_blank" >http://dx.doi.org/10.1111/ejh.12779</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/ejh.12779" target="_blank" >10.1111/ejh.12779</a>
Alternative languages
Result language
angličtina
Original language name
Activity of aldehyde dehydrogenase in B-cell and plasma cell subsets of monoclonal gammopathy patients and healthy donors
Original language description
Background: Aldehyde dehydrogenase (ALDH) is highly active in physiological stem cells as well as in tumor-initiating cells of some malignancies including multiple myeloma (MM). Finding higher activity of ALDH in some cell subsets in monoclonal gammopathies (MG) could identify potential source of myelomainitiating cells (MICs). Methods: Bone marrow of 12 MM, 9 monoclonal gammopathy of undetermined significance (MGUS), and 10 healthy donors (HD) were analyzed by flow cytometry. ALDH activity of B-cells and plasma cells (PC) was analyzed using Aldefluor. Results: Similar changes of ALDH activity were found during B-cell development in HD and MG. Decreasing of ALDH activity from immature to naive B-cells was found. In postgerminal stages, the activity started to increase, and in PCs, the ALDH activity was the same as in immature B-cells. Increased ALDH activity of all PC subsets compared to naive B-cells was found in MM as well as in HD, while in MGUS, only CD19(-)PCs have higher ALDH activity. In HD, ALDH activity was higher in CD19(+) PCs compared with MG. Conclusions: Our results indicate that changes of ALDH activity are the natural phenomenon in B-cell development; thus, high ALDH activity as a single marker is not appropriate for MICs identification.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30205 - Hematology
Result continuities
Project
<a href="/en/project/NV15-29508A" target="_blank" >NV15-29508A: Circulating nucleic acids as markers of multiple myeloma progression</a><br>
Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
EUROPEAN JOURNAL OF HAEMATOLOGY
ISSN
0902-4441
e-ISSN
1600-0609
Volume of the periodical
98
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
19-25
UT code for WoS article
000393166600002
EID of the result in the Scopus database
2-s2.0-84977142699