Comparison of two analytical methods for SARS-CoV-2 determination, RT-PCR vs. reverse transcription and loop-mediated isothermal amplification (RT-LAMP)

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17110%2F21%3AA2202DDJ" target="_blank" >RIV/61988987:17110/21:A2202DDJ - isvavai.cz</a>
Alternative codes found
RIV/00843989:_____/21:E0109165
Result on the web
<a href="https://www.cskb.cz/wp-content/uploads/2021/11/KBM_3_2021-Kusnierova.pdf" target="_blank" >https://www.cskb.cz/wp-content/uploads/2021/11/KBM_3_2021-Kusnierova.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Comparison of two analytical methods for SARS-CoV-2 determination, RT-PCR vs. reverse transcription and loop-mediated isothermal amplification (RT-LAMP)
Original language description
32.1 % of positive samples and 67.9 % of negative samples were detected by the RT-PCR method. In addition, 27.9 % of positive samples and 72.1 % of negative samples were detected by RT-LAMP method. Of the 327 positive samples identified by RT PCR, 247 samples were true positive, but 80 samples were false negative. It follows that the discrepancy of both tested methods was found to be 11.6 %. The RT-LAMP assay showed 94.50% specificity and 75.54% sensitivity. There was a substantial agreement between the two testing methods (k = 0.725).
Czech name
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Czech description
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Project
<a href="/en/project/FW01010052" target="_blank" >FW01010052: Development of new laboratory tests for the diagnosis of inflammation, sepsis and cardiovascular diseases based on chemiluminescence analysis on automated platforms</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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