Time- and temperature-dependent Pentraxin 3 stability in serum and bronchoalveolar lavage fluid samples

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17110%2F25%3AA2603BVO" target="_blank" >RIV/61988987:17110/25:A2603BVO - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/25:00637560 RIV/61989592:15110/25:73633210 RIV/61989592:15310/25:73633210 RIV/00098892:_____/25:10159339 and 2 more

Result on the web

<a href="https://academic.oup.com/mmy/article/doi/10.1093/mmy/myaf057/8176677" target="_blank" >https://academic.oup.com/mmy/article/doi/10.1093/mmy/myaf057/8176677</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/mmy/myaf057" target="_blank" >10.1093/mmy/myaf057</a>

Result language

angličtina

Original language name

Time- and temperature-dependent Pentraxin 3 stability in serum and bronchoalveolar lavage fluid samples

Original language description

Pentraxin 3 (Ptx3) is an acute-phase protein that specifically targets fungal galactosaminogalactan and has been proposed as a promising biomarker for invasive fungal infections. However, its stability in clinical samples over time has not yet been established. This study aimed to evaluate the stability of Ptx3 in serum and bronchoalveolar lavage fluid (BALF) samples during mid- and long-term storage. A total of 44 serum and 52 BALF samples were examined or re-examined for Ptx3 concentrations using enzyme immunoassay in pooled and individual sample formats. Samples were stored at −80°C, −20°C, and +37°C for periods ranging from 0 to 56 months. Statistical analyses included a paired two-sample Wilcoxon signed-rank test, analysis of variance, Bonferroni test, and linear regression analysis. Ptx3 remained highly stable in serum and BALF samples for up to 8 months at −20°C, with variations ranging from −1.8% to +2.8%. Long-term stability was observed at −80°C for 48 months, followed by a slow decline in Ptx3 levels. In contrast, storage at +37°C resulted in rapid degradation, with a 36.5%–60.7% increase or a 92.9%–97% decrease in Ptx3 levels in serum and BALF, respectively. These findings confirm that Ptx3 is a stable and reliable biomarker for invasive fungal infections when appropriate storage conditions are maintained.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30109 - Pathology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Medical Mycology

ISSN

1369-3786

e-ISSN

1460-2709

Volume of the periodical

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Issue of the periodical within the volume

7

Country of publishing house

GB - UNITED KINGDOM

Number of pages

9

Pages from-to

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UT code for WoS article

001525815700001

EID of the result in the Scopus database

2-s2.0-105010733754