Long-term stability of oxidative stress biomarkers in human serum
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F17%3A39910902" target="_blank" >RIV/00216275:25310/17:39910902 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1080/10715762.2017.1398403" target="_blank" >http://dx.doi.org/10.1080/10715762.2017.1398403</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/10715762.2017.1398403" target="_blank" >10.1080/10715762.2017.1398403</a>
Alternative languages
Result language
angličtina
Original language name
Long-term stability of oxidative stress biomarkers in human serum
Original language description
The storage time and storage temperature might affect stability of oxidative stress biomarkers, therefore, they have to be analyzed after long-term storage of serum samples. The stability of three biomarkers reflecting oxidative stress: reactive oxygen metabolites (ROM) for hydro peroxides, total thiol levels (TTL) for the redox status and biological antioxidant potency (BAP) for the antioxidant status, was investigated at several time points during 60 months of storage at -20 and -80°C. Biomarkers ROM and BAP showed a very good stability during storage for 60 months at both temperatures. In addition, the correlation of the data after 60 months of storage compared with the starting data was very good with correlation coefficients >0.9. The TTL assay showed good results in serum samples stored at -80°C, but not in samples stored at -20°C. Serum samples for analysis of the set of oxidative stress biomarkers ROM, BAP and TTL can be stored up to 60 months at -80°C. ROM and BAP can also be stored at -20°C during this period. The present results are very important for the biomarker-related epidemiological studies that make use of biobanks with samples stored for many years and for new project planning, including sample storage conditions.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30107 - Medicinal chemistry
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Free Radical Research
ISSN
1071-5762
e-ISSN
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Volume of the periodical
51
Issue of the periodical within the volume
11-12
Country of publishing house
GB - UNITED KINGDOM
Number of pages
8
Pages from-to
970-977
UT code for WoS article
000416652800008
EID of the result in the Scopus database
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