Analysis of Leishbuviridae from Trypanosomatids
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61988987%3A17310%2F25%3AA26039W2" target="_blank" >RIV/61988987:17310/25:A26039W2 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/10.1007/978-1-0716-4338-9_12" target="_blank" >https://link.springer.com/10.1007/978-1-0716-4338-9_12</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-0716-4338-9_12" target="_blank" >10.1007/978-1-0716-4338-9_12</a>
Alternative languages
Result language
angličtina
Original language name
Analysis of Leishbuviridae from Trypanosomatids
Original language description
Over the last decade, considerable progress has been made in unraveling RNA virus diversity. This has contributed to our understanding of the evolution of these viruses, which include emerging zoonotic human pathogens. Current success has been greatly facilitated by the development of next-generation sequencing platforms instrumental for meta-transcriptomic studies. However, due to the rapid evolution of RNA viruses, there are numerous "blind spots" waiting to be explored; one of those is the RNA virome of unicellular eukaryotes. Here, we present the pipeline, which has been successfully used to characterize various types of RNA viruses, including Leishbuviridae (Bunyaviricetes, Hareavirales) in the parasitic flagellates of the family Trypanosomatidae. The pipeline relies on axenic in vitro cell culture and double-stranded RNA enrichment, followed by direct RNA-sequencing. A detailed procedure description starting from the initial total RNA preparation to the final assembly of the viral segments is provided.
Czech name
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Czech description
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Classification
Type
C - Chapter in a specialist book
CEP classification
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OECD FORD branch
10607 - Virology
Result continuities
Project
<a href="/en/project/GA24-10009S" target="_blank" >GA24-10009S: Viruses of trypanosomatids: maintenance and transmission</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
Bunyaviruses
ISBN
978-1-0716-4338-9
Number of pages of the result
17
Pages from-to
151-167
Number of pages of the book
301
Publisher name
Humana
Place of publication
New York
UT code for WoS chapter
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