Low-cost optical fiber sensors for the portable detection of cortisol and E. coli
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989100%3A27240%2F25%3A10257794" target="_blank" >RIV/61989100:27240/25:10257794 - isvavai.cz</a>
Result on the web
<a href="https://opg.optica.org/oe/fulltext.cfm?uri=oe-33-9-19851&id=570772" target="_blank" >https://opg.optica.org/oe/fulltext.cfm?uri=oe-33-9-19851&id=570772</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1364/OE.558813" target="_blank" >10.1364/OE.558813</a>
Alternative languages
Result language
angličtina
Original language name
Low-cost optical fiber sensors for the portable detection of cortisol and E. coli
Original language description
Detection of biomarkers, as well as bacterial infection levels, plays an important role in clinical analysis. Development in research for fast, high-sensitivity, and selective sensors has a key role in decision-making during a health issue. Aligning to these features, low-cost biomarkers and bacteria population detection methods should be developed in order to make clinical trials more accessible to the whole of society. In this context, optical sensors based on surface plasmon resonance (SPR) phenomena have been intensively studied due to their easy functionalization and fast and amplified response. This work aimed at the optical detection of some analytes in aqueous solutions. SPR-based D-shape sensors employing gold-deposited polymer optical fibers (POF) were used in a portable platform to detect concentrations of cortisol, the stress hormone, and E. coli, a bacteria strain. The sensors showed good linearity in response to increasing cortisol concentrations, but were less consistent in detecting the E. coli bacteria. However, E. coli sensors possess a sensitivity of-0.38 +/- 0.06 nm/log(CFU/mL), bearing a resemblance to the sensitivity obtained for cortisol sensors with-0.41 +/- 0.04 nm/log(ng/mL). The biosensors had limits of detection (LODs) lower than those of existing literature, with E. coli detection of 3.72 x 10-7 CFU/mL, and cortisol detection of 1.73 x 10-4 pg/mL. Small topography profile differences of about 2.0-3.4 nm were found through atomic force microscopy (AFM), equivalent to the thickness of the used antibodies, suggesting they were deposited parallel to the gold surface. In the future, methods with the potential to assure antibody verticality should be explored.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10306 - Optics (including laser optics and quantum optics)
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Optics Express
ISSN
1094-4087
e-ISSN
1094-4087
Volume of the periodical
33
Issue of the periodical within the volume
9
Country of publishing house
US - UNITED STATES
Number of pages
14
Pages from-to
19851-19864
UT code for WoS article
001487064300002
EID of the result in the Scopus database
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