Biological response of an in vitro human 3D lung cell model exposed to brake wear debris varies based on brake pad formulation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989100%3A27640%2F18%3A10238892" target="_blank" >RIV/61989100:27640/18:10238892 - isvavai.cz</a>

Result on the web

<a href="https://link.springer.com/article/10.1007%2Fs00204-018-2218-8" target="_blank" >https://link.springer.com/article/10.1007%2Fs00204-018-2218-8</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00204-018-2218-8" target="_blank" >10.1007/s00204-018-2218-8</a>

Result language

angličtina

Original language name

Biological response of an in vitro human 3D lung cell model exposed to brake wear debris varies based on brake pad formulation

Original language description

Wear particles from automotive friction brake pads of various sizes, morphology, and chemical composition are significant contributors towards particulate matter. Knowledge concerning the potential adverse effects following inhalation exposure to brake wear debris is limited. Our aim was, therefore, to generate brake wear particles released from commercial low-metallic and non-asbestos organic automotive brake pads used in mid-size passenger cars by a full-scale brake dynamometer with an environmental chamber simulating urban driving and to deduce their potential hazard in vitro. The collected fractions were analysed using scanning electron microscopy via energy-dispersive X-ray spectroscopy (SEM-EDS) and Raman microspectroscopy. The biological impact of the samples was investigated using a human 3D multicellular model consisting of human epithelial cells (A549) and human primary immune cells (macrophages and dendritic cells) mimicking the human epithelial tissue barrier. The viability, morphology, oxidative stress, and (pro-)inflammatory response of the cells were assessed following 24 h exposure to ~ 12, ~ 24, and ~ 48 µg/cm2 of non-airborne samples and to ~ 3.7 µg/cm2 of different brake wear size fractions (2-4, 1-2, and 0.25-1 µm) applying a pseudo-air-liquid interface approach. Brake wear debris with low-metallic formula does not induce any adverse biological effects to the in vitro lung multicellular model. Brake wear particles from non-asbestos organic formulated pads, however, induced increased (pro-)inflammatory mediator release from the same in vitro system. The latter finding can be attributed to the different particle compositions, specifically the presence of anatase.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

21001 - Nano-materials (production and properties)

Project

—

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Archives of Toxicology

ISSN

0340-5761

e-ISSN

—

Volume of the periodical

92

Issue of the periodical within the volume

7

Country of publishing house

DE - GERMANY

Number of pages

13

Pages from-to

2339-2351

UT code for WoS article

000436105400015

EID of the result in the Scopus database

2-s2.0-85046804076