Batch immunoextraction method for an effective purification of aromatic cytokinins.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F05%3A00001951" target="_blank" >RIV/61989592:15110/05:00001951 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Batch immunoextraction method for an effective purification of aromatic cytokinins.
Original language description
Arange of benzylaminopurines naturally occur in plants and exhibit high biological activity. Others have been synthesized, such as 6-(2-hydroxy-3-methoxybenzylamino)purine riboside (2OH3MeOBAPR), which has shown interesting anti-cancer activity under invitro conditions. In orderto study the biological activity of this interesting compound in more detail, a rapid and highly efficient method for its purification from complexsamples (e.g. blood and plant extracts) is needed. Therefore, we prepared monoclonal antibodies against 2OH3MeOBAPR. The antibody hadundetectable cross-reactivity with all natural isoprenoid cytokinins, but relatively high cross-reactivity with aromatic cytokinins as well as somesynthetic di- and tri-substituted 6-benzylaminopurinesand the corresponding ribosides. The antibody also showed strong responses and specificityin enzyme-linked immunoassays (ELISAs). In addition, it was used to prepare, for the first time, an immunoaffinity sorbent with high specificityand
Czech name
Série imunoextrakčních metod na efektivní purifikaci aromatických cytokininů.
Czech description
Přirozené benzylaminopuriny přitomné v rostlinách mají vysokou bilogickou aktivitu. Další byly syntetizované jak npř. 6-(2-hydroxy-3-methoxybenzylamino)purine riboside (2OH3MeOBAPR), které vykazují vysokou protinádorovou aktivitu v in vitro podmínkách.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FD - Oncology and haematology
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2005
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Chromatography A: Symposium Volumes
ISSN
0021-9673
e-ISSN
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Volume of the periodical
1100
Issue of the periodical within the volume
1
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
10
Pages from-to
116-125
UT code for WoS article
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EID of the result in the Scopus database
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