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New benzylaminopurine derivatives - monoclonal antibody preparation and immunoaffinity chromatography development

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F04%3A00002312" target="_blank" >RIV/61989592:15310/04:00002312 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    New benzylaminopurine derivatives - monoclonal antibody preparation and immunoaffinity chromatography development

  • Original language description

    Benzylaminopurines naturally occur in plants and exhibit high cytokinin activity. Synthetic compound 6-(2-hydroxy-3-methoxybenzylamino)purine riboside (2-OH-3-OCH3-BAPR) structurally also belongs to this group. Surprisingly, we have proved its high cytotoxicity against CEM and HL-60 leukaemia cell lines. To study different aspects of biological activity of this interesting compound in more details, rapid and highly efficient method for its purification from complex samples (blood, plant extracts) is needed. Therefore, we have prepared monoclonal antibodies against 2-OH-3-OCH3-BAPR, which have been subsequently utilized for preparation of immunoaffinity sorbent and columns.

  • Czech name

    Nové deriváty benzylaminopurinu - příprava monoklonálních protilátek a vývoj imunoafinitní chromatografie

  • Czech description

    Článek se zabývá novými deriváty benzylaminopurinu - přípravou monoklonálních protilátek a vývojem imunoafinitní chromatografie.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EF - Botany

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/KJB6137301" target="_blank" >KJB6137301: 8-Substituted Analogues of Cytokinins - Utilization of their Potential New Quality in Plant Research</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2004

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Acta Physiologiae Plantarum

  • ISSN

    0137-5881

  • e-ISSN

  • Volume of the periodical

    26

  • Issue of the periodical within the volume

    Special

  • Country of publishing house

    PL - POLAND

  • Number of pages

    318

  • Pages from-to

    40

  • UT code for WoS article

  • EID of the result in the Scopus database