McRAPD as a new approach to rapid and accurate identification of pathogenic yeasts
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F05%3A00002046" target="_blank" >RIV/61989592:15110/05:00002046 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
McRAPD as a new approach to rapid and accurate identification of pathogenic yeasts
Original language description
Despite advances in antifungal prophylaxis and therapy, morbidity and mortality incurred by yeasts remain a significant burden. As pathogenic yeast species vary in their susceptibilities to antifungal agents, clinical microbiology laboratories face an important challenge to identify them rapidly and accurately. Although a vast array of phenotyping and genotyping methods has been developmed these are either unable to cover the whole spectrum of potential yeast pathogens or can do this only in a rather costly or laborious way. Random amplified polymorphic DNA (RAPD) fingerprinting was repeatedly demonstrated to be a convenient tool for species identification in pathogenic yeasts. However, its wider acceptance has been limited mainly due to special expertise and software needed for analysis and comparison of the resulting banding patterns. Based on a pilot study, we demonstrate here that a simple and rapid melting curve analysis of RAPD products can provide data for identification of five
Czech name
McRAPD as a new approach to rapid and accurate identification of pathogenic yeasts
Czech description
Náhodná aplifikace polymorfní DNA (RAPD) se opakovaně ukázala jako vhodný nástroj pro druhovou identifikaci patogenních kvasinek. Na základě pilotní studie demonstrujeme, že jednoduchá a rychlá metoda analýzy teplotní křivky RAPD produktů umožňuje identifikaci pěti lékařsky nejvýznamnějších druhů kandid.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/NI7498" target="_blank" >NI7498: Rapid PCR detection and epidemiology of nosocomial invasive aspergillosis</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2005
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Microbiological Methods
ISSN
0167-7012
e-ISSN
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Volume of the periodical
60
Issue of the periodical within the volume
1
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
7
Pages from-to
107-113
UT code for WoS article
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EID of the result in the Scopus database
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