ATR-Chk1-APC/CCdh1-dependent stabilization of Cdc7-ASK (Dbf4) kinase is required for DNA lesion bypass under replication stress
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F13%3A33145215" target="_blank" >RIV/61989592:15110/13:33145215 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1101/gad.224568.113" target="_blank" >http://dx.doi.org/10.1101/gad.224568.113</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1101/gad.224568.113" target="_blank" >10.1101/gad.224568.113</a>
Alternative languages
Result language
angličtina
Original language name
ATR-Chk1-APC/CCdh1-dependent stabilization of Cdc7-ASK (Dbf4) kinase is required for DNA lesion bypass under replication stress
Original language description
Cdc7 kinase regulates DNA replication. However, its role in DNA repair and recombination is poorly understood. Here we describe a pathway that stabilizes the human Cdc7-ASK (activator of S-phase kinase; also called Dbf4), its regulation, and its functionin cellular responses to compromised DNA replication. Stalled DNA replication evoked stabilization of the Cdc7-ASK (Dbf4) complex in a manner dependent on ATR-Chk1-mediated checkpoint signaling and its interplay with the anaphase-promoting complex/cyclosome(Cdh1) (APC/C-Cdh1) ubiquitin ligase. Mechanistically, Chk1 kinase inactivates APC/C-Cdh1 through degradation of Cdh1 upon replication block, thereby stabilizing APC/C-Cdh1 substrates, including Cdc7-ASK (Dbf4). Furthermore, motif C of ASK (Dbf4) interacts with the N-terminal region of RAD18 ubiquitin ligase, and this interaction is required for chromatin binding of RAD18. Impaired interaction of ASK (Dbf4) with RAD18 disables foci formation by RAD18 and hinders chromatin loading of
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FD - Oncology and haematology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Genes & Development
ISSN
0890-9369
e-ISSN
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Volume of the periodical
27
Issue of the periodical within the volume
22
Country of publishing house
US - UNITED STATES
Number of pages
14
Pages from-to
2459-2472
UT code for WoS article
000327321300007
EID of the result in the Scopus database
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