FBH1 Catalyzes Regression of Stalled Replication Forks

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F15%3A33158100" target="_blank" >RIV/61989592:15110/15:33158100 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1016/j.celrep.2015.02.028" target="_blank" >http://dx.doi.org/10.1016/j.celrep.2015.02.028</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.celrep.2015.02.028" target="_blank" >10.1016/j.celrep.2015.02.028</a>

Result language

angličtina

Original language name

FBH1 Catalyzes Regression of Stalled Replication Forks

Original language description

DNA replication fork perturbation is a major challenge to the maintenance of genome integrity. It has been suggested that processing of stalled forks might involve fork regression, in which the fork reverses and the two nascent DNA strands anneal. Here,we show that FBH1 catalyzes regression of a model replication fork in vitro and promotes fork regression in vivo in response to replication perturbation. Cells respond to fork stalling by activating checkpoint responses requiring signaling through stress-activated protein kinases. Importantly, we show that FBH1, through its helicase activity, is required for early phosphorylation of ATM substrates such as CHK2 and CtIP as well as hyperphosphorylation of RPA. These phosphorylations occur prior to apparent DNA double-strand break formation. Furthermore, FBH1-dependent signaling promotes checkpoint control and preserves genome integrity. We propose a model whereby FBH1 promotes early checkpoint signaling by remodeling of stalled DNA replic

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cell Reports

ISSN

2211-1247

e-ISSN

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Volume of the periodical

10

Issue of the periodical within the volume

10

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

1749-1757

UT code for WoS article

000351316700011

EID of the result in the Scopus database

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