Defining HIV-1 Envelope N-Glycan Microdomains through Site-Specific Heterogeneity Profiles

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F19%3A73589832" target="_blank" >RIV/61989592:15110/19:73589832 - isvavai.cz</a>

Result on the web

<a href="https://jvi.asm.org/content/93/1/e01177-18.long" target="_blank" >https://jvi.asm.org/content/93/1/e01177-18.long</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1128/JVI.01177-18" target="_blank" >10.1128/JVI.01177-18</a>

Result language

angličtina

Original language name

Defining HIV-1 Envelope N-Glycan Microdomains through Site-Specific Heterogeneity Profiles

Original language description

The HIV-1 envelope (Env) glycans shield the surface of Env from the immune system and form integral interactions important for a functional Env. To understand how individual N-glycosylation sites (NGS) coordinate to form a dynamic shield and evade the immune system through mutations, we tracked 20 NGS in Env from HIV-transmitted/founder (T/F) and immune escape variants and their mutants involving the N262 glycan. NGS were profiled in a site-specific manner using a high-resolution mass spectrometry (MS)-based workflow. Using this site-specific quantitative heterogeneity profiling, we empirically characterized the interdependent NGS of a microdomain in the high-mannose patch (HMP). The changes (shifts) in NGS heterogeneity between the T/F and immune escape variants defined a range of NGS that we further probed for exclusive combinations of sequons in the HMP microdomain using the Los Alamos National Laboratory HIV sequence database. The resultant sequon combinations, including the highly conserved NGS N262, N448, and N301, created an immune escape map of the conserved and variable sequons in the HMP microdomain. This report provides details on how some clustered NGS form microdomains that can be identified and tracked across Env variants. These microdomains have a limited number of N-glycan-sequon combinations that may allow the anticipation of immune escape variants.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30102 - Immunology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

JOURNAL OF VIROLOGY

ISSN

0022-538X

e-ISSN

—

Volume of the periodical

93

Issue of the periodical within the volume

1

Country of publishing house

US - UNITED STATES

Number of pages

20

Pages from-to

"e01177"-18

UT code for WoS article

000454918700005

EID of the result in the Scopus database

2-s2.0-85058472075