Glycan Positioning Impacts HIV-1 Env Glycan-Shield Density, Function, and Recognition by Antibodies
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F20%3A73602949" target="_blank" >RIV/61989592:15110/20:73602949 - isvavai.cz</a>
Result on the web
<a href="https://www.cell.com/action/showPdf?pii=S2589-0042%2820%2930908-1" target="_blank" >https://www.cell.com/action/showPdf?pii=S2589-0042%2820%2930908-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.isci.2020.101711" target="_blank" >10.1016/j.isci.2020.101711</a>
Alternative languages
Result language
angličtina
Original language name
Glycan Positioning Impacts HIV-1 Env Glycan-Shield Density, Function, and Recognition by Antibodies
Original language description
HIV-1 envelope (Env) N-glycosylation impact virus-cell entry and immune evasion. How each glycan interacts to shape the Env-protein-sugar complex and affects Env function is not well understood. Here, analysis of two Env variants from the same donor, with differing functional characteristics and N-glycosylation-site composition, revealed that changes to key N-glycosylation sites affected the Env structure at distant locations and had a ripple effect on Env-wide glycan processing, virus infectivity, antibody recognition, and virus neutralization. Specifically, the N262 glycan, although not in the CD4-binding site, modulated Env binding to the CD4 receptor, affected Env recognition by several glycan-dependent neutralizing antibodies, and altered site-specific glycosylation heterogeneity, with, for example, N448 displaying limited glycan processing. Molecular-dynamic simulations visualized differences in glycan density and how specific oligosaccharide positions can move to compensate for a glycan loss. This study demonstrates how changes in individual glycans can alter molecular dynamics, processing, and function of the Env-glycan shield.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30102 - Immunology
Result continuities
Project
<a href="/en/project/EF16_025%2F0007397" target="_blank" >EF16_025/0007397: Recombinant Biotechnology and Immunotherapy Centre</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
iScience
ISSN
2589-0042
e-ISSN
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Volume of the periodical
22
Issue of the periodical within the volume
11
Country of publishing house
US - UNITED STATES
Number of pages
35
Pages from-to
"'101711(1)'"-"'101711(35)'"
UT code for WoS article
000596692100003
EID of the result in the Scopus database
2-s2.0-85094969909