Cell membrane nanostructure is altered by heat-induced antigen retrieval:A possible consequence for immunocytochemical detection of membranous antigens
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F20%3A73599480" target="_blank" >RIV/61989592:15110/20:73599480 - isvavai.cz</a>
Result on the web
<a href="https://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/abs/cell-membrane-nanostructure-is-altered-by-heatinduced-antigen-retrieval-a-possible-consequence-for-immunocytochemical-detection-of-membranous-antigens/2B3F902B8BD819D22B91F8D08718A41D" target="_blank" >https://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/abs/cell-membrane-nanostructure-is-altered-by-heatinduced-antigen-retrieval-a-possible-consequence-for-immunocytochemical-detection-of-membranous-antigens/2B3F902B8BD819D22B91F8D08718A41D</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1017/S1431927619015113" target="_blank" >10.1017/S1431927619015113</a>
Alternative languages
Result language
angličtina
Original language name
Cell membrane nanostructure is altered by heat-induced antigen retrieval:A possible consequence for immunocytochemical detection of membranous antigens
Original language description
Heat-induced antigen retrieval (HIAR) improves immunodetection of antigens in formalin-fixed, paraffin-embedded tissue samples and it can also improve detection especially of intracellular antigens in alcohol-fixed cytological samples although it can have deleterious effect especially for membranous antigens. We examined the differences in cell surface topography by atomic force microscopy on methanol:aceton- (M:A) and 4% paraformaldehyde (4% PFA)-fixed MCF7 cells and changes caused by HIAR at three different temperatures (60, 90, 120°C). Moreover, the consequences for immunostaining of five membranous antigens (EGFR, E-cadherin, CD9, CD24 and CD44) were examinated. Our result showed that albeit there is no optimal immunostaining condition for our tested antibodies, the surface topography could be one of the important factors. Generally, the best conditions for successful immunostaining are M:A fixation with no HIAR treatment whereas in 4% PFA-fixed cells HIAR 120 is optimal. These conditions showed similarity in cell surface skewness which indicates with correlation factor 0.8000 between successful ICC staining and skewness parameter. It indicates that presence of valleys, depressions, scratches and pits on cell surface represents unfavourable condition for successful immunodetection of cell surface antigens.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30106 - Anatomy and morphology (plant science to be 1.6)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Microscopy and Microanalysis
ISSN
1431-9276
e-ISSN
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Volume of the periodical
26
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
139-147
UT code for WoS article
000516841400017
EID of the result in the Scopus database
2-s2.0-85080827035