HT - 29 and Caco 2 cell lines are suitable models for studying the role of arachidonic acid - metabolizing enzymes in intestinal cell differentiation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F20%3A73604399" target="_blank" >RIV/61989592:15110/20:73604399 - isvavai.cz</a>

Result on the web

<a href="https://www.karger.com/Article/FullText/506735" target="_blank" >https://www.karger.com/Article/FullText/506735</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1159/000506735" target="_blank" >10.1159/000506735</a>

Result language

angličtina

Original language name

HT - 29 and Caco 2 cell lines are suitable models for studying the role of arachidonic acid - metabolizing enzymes in intestinal cell differentiation

Original language description

Introduction: Cytochrome (CYP) epoxygenases (CYP2C and CYP2J) and soluble epoxide hydrolase (sEH) participate in the metabolism of arachidonic acid and may also have a potential role in enterocyte differentiation. The first critical step in the study of intestinal cell differentiation is the determinationof a suitable in vitro model, which must be as similar as possible to the conditions of a living organism. It is known that HT-29 and Caco2 cell lines derived from human colorectal carcinomas can differentiate into enterocyte-like cells in appropriate culture conditions. Material and Methods: We tested 4 different approaches of enterocyte-like differentiation and determined the most appropriate culture conditions for each model. Subsequently, the changes in the expression of CYP epoxygenases and sEH in undifferentiated and differentiated cells were measured by In-Cell ELISA. These results were compared with immunohistochemical profiles of expression of CYP epoxygenases and sEH in samples of human embryonic and fetal intestines as well as adult duodenum and colon. Results: Our results show that sodium butyrate (NaBt)-differentiated HT-29 cells and spontaneously differentiated Caco2 cells resemble CYP epoxygenases and sEH profiles, corresponding with different types of intestines. Conclusion: Our study revealed that the most suitable models for the study of the role of CYP epoxygenases and sEH expression in differentiation of intestinal epithelium are NaBt-differentiated HT-29 cells and spontaneously differentiated Caco2 cells.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30109 - Pathology

Project

<a href="/en/project/LM2015062" target="_blank" >LM2015062: National Infrastructure for Biological and Medical Imaging</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2020

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

CELLS TISSUES ORGANS

ISSN

1422-6405

e-ISSN

—

Volume of the periodical

208

Issue of the periodical within the volume

1-2

Country of publishing house

CH - SWITZERLAND

Number of pages

11

Pages from-to

37-47

UT code for WoS article

000531372100004

EID of the result in the Scopus database

2-s2.0-85083184407