A study on the reactions of plant copper amine oxidase with C3 and C4 aliphatic diamines

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F00%3A00001108" target="_blank" >RIV/61989592:15310/00:00001108 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15310/00:00007755

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

A study on the reactions of plant copper amine oxidase with C3 and C4 aliphatic diamines

Original language description

The paper reports a study on the reactions of grass pea (Lathyrus sativus) amine oxidase (GPAO) with several aliphatic diamines. The influence of the chain length and of unsaturations in the molecules was examined. Kinetic measurements confirmed that trans- i.e. (E)-2-butene-1,4-diamine (TDABE) and cis- i.e. (Z)-2-butene-1,4-diamine (CDABE) could be classified as good substrates. Propane-1,3-diamine (DAP) and propene-1,3-diamine (DAPE) were only weakly oxidized, whereas 1,3-diamino-2-propanol (DAPL) wasnot utilized as a substrate. Contrary to the inactivator 2-butyne-1,4-diamine (DABI), DAPE was shown to be only a competitive inhibitor. DAP itself did not inhibit the catalytic activity. Irreversible inhibition of the activity occurred only after the incubation of GPAO with DABI; other diamines were without this effect. Differential pulse polarography and chromatofocusing confirmed that the aminoaldehyde product of DABI oxidation binds to the enzyme. Activity assay of pea aminoaldehyde

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2000

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Archives of Biochemistry and Biophysics

ISSN

0003-9861

e-ISSN

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Volume of the periodical

384

Issue of the periodical within the volume

1

Country of publishing house

XX - stateless person

Number of pages

12

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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