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Biochemistry of cytokinin dehydrogenase.

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F04%3A00002099" target="_blank" >RIV/61989592:15310/04:00002099 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Biochemistry of cytokinin dehydrogenase.

  • Original language description

    Degradation of cytokinins in plants is controlled by flavoprotein cytokinin dehydrogenase (EC 1.5.99.12). Kinetic measurements and stopped-flow spectrophotometry on a recombinant enzyme from maize (ZmCKX1) revealed that the cleavage of cytokinin substrates proceeds via ternary complex involving FAD cofactor and a quinonic electron acceptor (2,3-dimethoxy-5-methyl-1,4-benzoquinone as a model compound). Results of site-directed mutagenesis and structural homology modeling disclosed C-terminus of the protein as an important domain for functional conformation of the enzyme, with the covalently bound FAD cofactor being buried in a hydrophobic pocket. Functional electron acceptors of ZmCKX1 were generated in vitro from acetosyringone and some catechols and flavonoids by conversion either with peroxidase/hydrogen peroxide system or mushroom tyrosinase (a commercial substitute for plant catechol oxidase/laccase). Histochemical localizations in maize kernel showed co-staining of cytokinin dehyd

  • Czech name

    Biochemie cytokinindehydrogenasy

  • Czech description

    Příspěvek se věnuje biochemii cytokinindehydrogenasy.

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    CE - Biochemistry

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GA522%2F03%2F0979" target="_blank" >GA522/03/0979: Organization of cytokinin dehydrogenase genes in cereals and mechanism of the catalytic reaction of cytokinin degradation</a><br>

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2004

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    Conference Handbook, 18th International Conference on Plant Growth Substances

  • ISBN

  • ISSN

  • e-ISSN

  • Number of pages

    144

  • Pages from-to

    55

  • Publisher name

    IPGSA

  • Place of publication

    Canberra, Australia

  • Event location

  • Event date

  • Type of event by nationality

  • UT code for WoS article