Changes in the amount of available grapevine DNA during vinification

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F06%3A00003071" target="_blank" >RIV/61989592:15310/06:00003071 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Changes in the amount of available grapevine DNA during vinification

Original language description

In this paper, we attempted to challenge common view that grapevine DNA is either released in low amounts from grapes or completely lost during vinification. We followed concentration of grapevine DNA by PicoGreen fluorimetry and by quantitative PCR withTaqMan probe in samples obtained during vinifications of wines from four grapevine varieties: white wines Welsh Riesling and Frühroter Veltliner, rosé wine Saint Laurent, and red wine Blaufrankisch.We found that amount of DNA extracted depends on DNA extraction method, grapevine variety, vinification process, and timing of sampling. Based on our data, tens of genome equivalents of grapevine DNA can be extracted from a single wine bottle. Thus, DNA profiling might be possible even from wine

Czech name

Změny v množství dostupné vinné DNA v průběhu přípravy vína

Czech description

V tomto clanku si dovolujeme zpochybnit nazor, ze behem pripravy vina je revova DNA bud uvolnena v nedetekovatelnem mnozstvi, anebo uplne ztracena. Merili jsme koncentraci DNA v prubehu vinifikace ctyr odrudovych vin (Ryzlink vlassky, Veltlinske cervenerane, Svatovavrinecke, Frankovka) pomoci PicoGreen fluorimetrie a kvantitativni PCR s TaqMan sondou. Objevili jsme, ze mnozstvi extrahovane DNA zavisi na izolacni metode, odrude revy, priprave vina a casu odberu vzorku. Podle nasich udaju je z kazde lahve vina ziskatelnych nekolik desitek ekvivalentu revoveho genomu, a tak se zda DNA profilovani z vina mozne.

Type

D - Article in proceedings

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/1K04104" target="_blank" >1K04104: UP-Drábek: Typing of degraded DNA of Sambucus nigra and Vitis vinifera using molecular genetics methods</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Proceedings of the AMPELOS 2006 International Symposium on the evaluation and exploitation of grapes of corresponding terroir through winemaking and commercialization of wines

ISBN

960-89228-0-1

ISSN

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e-ISSN

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Number of pages

240

Pages from-to

23-26

Publisher name

Heliotopos Conferences Ltd.

Place of publication

Athens, Greece

Event location

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Event date

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Type of event by nationality

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UT code for WoS article

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