Reaction pathways involved in the production of hydroxyl radicals in thylakoid membrane: EPR spin-trapping study.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F06%3A00003237" target="_blank" >RIV/61989592:15310/06:00003237 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Reaction pathways involved in the production of hydroxyl radicals in thylakoid membrane: EPR spin-trapping study.
Original language description
It has been suggested that both free metals and reduced ferredoxin (Fd) participate in the light-induced production of hydroxyl radicals (OH.) in thylakoid membranes of chloroplasts. The most direct evidence of the involvement of Fd in OH. formation under physiological conditions was reported by Jakob and Heber (Plant Cell Physiol., 1996, 37, 629-635), who used the oxidation of dimethylsulfoxide to methane sulfinic acid as an indicator of OH. production. We confirmed their conclusions using a more sensitive and reliable EPR spin-trapping method and extended their work by additional findings. Free metal-dependent and ferredoxin-dependent OH. production was studied simultaneously and strong metal chelator Desferal was used to distinguish between these reaction pathways. The participation of protein-bound iron within photosystem I was confirmed by partial suppression of OH. generation in broken chloroplasts by methyl viologen. The stimulation of the production of OH. in thylakoid membrane
Czech name
Reakční cesty produkce hydroxylových radikálů v thylakoidní membráně: spin-trapping EPR studie.
Czech description
It has been suggested that both free metals and reduced ferredoxin (Fd) participate in the light-induced production of hydroxyl radicals (OH.) in thylakoid membranes of chloroplasts. The most direct evidence of the involvement of Fd in OH. formation under physiological conditions was reported by Jakob and Heber (Plant Cell Physiol., 1996, 37, 629-635), who used the oxidation of dimethylsulfoxide to methane sulfinic acid as an indicator of OH. production. We confirmed their conclusions using a more sensitive and reliable EPR spin-trapping method and extended their work by additional findings. Free metal-dependent and ferredoxin-dependent OH. production was studied simultaneously and strong metal chelator Desferal was used to distinguish between these reaction pathways. The participation of protein-bound iron within photosystem I was confirmed by partial suppression of OH. generation in broken chloroplasts by methyl viologen. The stimulation of the production of OH. in thylakoid membrane
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Result continuities
Project
<a href="/en/project/1K04103" target="_blank" >1K04103: Reactive oxygen species in photosynthesis</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2006
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Photochemical and Photobiological Sciences
ISSN
1474-905X
e-ISSN
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Volume of the periodical
5
Issue of the periodical within the volume
5
Country of publishing house
GB - UNITED KINGDOM
Number of pages
5
Pages from-to
472-476
UT code for WoS article
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EID of the result in the Scopus database
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