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Reaction pathways involved in the production of hydroxyl radicals in thylakoid membrane: EPR spin-trapping study.

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F06%3A00003237" target="_blank" >RIV/61989592:15310/06:00003237 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Reaction pathways involved in the production of hydroxyl radicals in thylakoid membrane: EPR spin-trapping study.

  • Original language description

    It has been suggested that both free metals and reduced ferredoxin (Fd) participate in the light-induced production of hydroxyl radicals (OH.) in thylakoid membranes of chloroplasts. The most direct evidence of the involvement of Fd in OH. formation under physiological conditions was reported by Jakob and Heber (Plant Cell Physiol., 1996, 37, 629-635), who used the oxidation of dimethylsulfoxide to methane sulfinic acid as an indicator of OH. production. We confirmed their conclusions using a more sensitive and reliable EPR spin-trapping method and extended their work by additional findings. Free metal-dependent and ferredoxin-dependent OH. production was studied simultaneously and strong metal chelator Desferal was used to distinguish between these reaction pathways. The participation of protein-bound iron within photosystem I was confirmed by partial suppression of OH. generation in broken chloroplasts by methyl viologen. The stimulation of the production of OH. in thylakoid membrane

  • Czech name

    Reakční cesty produkce hydroxylových radikálů v thylakoidní membráně: spin-trapping EPR studie.

  • Czech description

    It has been suggested that both free metals and reduced ferredoxin (Fd) participate in the light-induced production of hydroxyl radicals (OH.) in thylakoid membranes of chloroplasts. The most direct evidence of the involvement of Fd in OH. formation under physiological conditions was reported by Jakob and Heber (Plant Cell Physiol., 1996, 37, 629-635), who used the oxidation of dimethylsulfoxide to methane sulfinic acid as an indicator of OH. production. We confirmed their conclusions using a more sensitive and reliable EPR spin-trapping method and extended their work by additional findings. Free metal-dependent and ferredoxin-dependent OH. production was studied simultaneously and strong metal chelator Desferal was used to distinguish between these reaction pathways. The participation of protein-bound iron within photosystem I was confirmed by partial suppression of OH. generation in broken chloroplasts by methyl viologen. The stimulation of the production of OH. in thylakoid membrane

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    BO - Biophysics

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/1K04103" target="_blank" >1K04103: Reactive oxygen species in photosynthesis</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2006

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Photochemical and Photobiological Sciences

  • ISSN

    1474-905X

  • e-ISSN

  • Volume of the periodical

    5

  • Issue of the periodical within the volume

    5

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    5

  • Pages from-to

    472-476

  • UT code for WoS article

  • EID of the result in the Scopus database