HPLC determination of lincomycin in premixes and feedstuffs with solid-phase extraction on HLB OASIS and LC-MS/MS confirmation
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F06%3A00003365" target="_blank" >RIV/61989592:15310/06:00003365 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
HPLC determination of lincomycin in premixes and feedstuffs with solid-phase extraction on HLB OASIS and LC-MS/MS confirmation
Original language description
A rapid clean-up procedure based on solid-phase extraction (SPE) and HPLC determination of lincomycin in premixes with UV detection is described. After extraction of lincomycin from premix with extraction solvent the extract is applied to OASIS HLB column treated with methanol and water. Lincomycin is eluted with methanol and effluent is analysed on analytical column (phenyl) using mobile phase consists 0.2% phosphoric acid in water and acetonitrile (875:125, v/v). Detection is performed at 208 nm. Quantitation is carried out using external standard. The mean recovery of lincomycin was 105.0 +/- 7.3%, in concentration range of 250-750 mg k,(-1), and 99.8 +/- 3.7%, in concentration range of 10,000-150,000mg kg(-1). The limit of determination, based on asignal-to-noise ratio of 10:1, was 5.2mg kg(-1). LC-MS/MS confirmation of lincomycin is also presented. Identification was performed by monitoring two pairs of multiple reaction monitoring ions from the parent ions (m/z 407.2 -> 126.1 an
Czech name
HPLC stanovení linkomycinu v premixech a krmných směsích s použitím solid-phase extrakce na HLB OASIS a LC-MS/MS potvrzení
Czech description
Rychlá procedura založená na solid-phase extrakci a HPLC stanovení linkomycinu v premixech s UV detekcí je v práci popsána. Po extrakci linkomycinu pomocí extrakčního činidla je extrakt nastříknut na OASIS HLB kolonu. Linkomycin je eluován metanolem a eluent je analyzován na analytické koloně s použitím mobilní fáze složené z 0,2 % kyseliny fosforečné ve směsi vody a acetonitrilu. Detekce je prováděna při 208 nm.Ke kvantifikaci se používá metoda vnějšího standardu. Průměrná výtěžnost byla 105,0 +/- 7,3% v koncentračním rozmezí 250-750 mg/kg a 99,8 +/- 3,7 % v koncentračním rozmezí 10000-150000 mg/kg. Mez detekce určená poměrem signál ku šumu 10:1 byla 5,2 mg/kg. LC-MS/MS konfirmace linkomycinu je také prezentována.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2006
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Pharmaceutical and Biomedical Analysis
ISSN
0731-7085
e-ISSN
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Volume of the periodical
40
Issue of the periodical within the volume
4
Country of publishing house
GB - UNITED KINGDOM
Number of pages
6
Pages from-to
981-986
UT code for WoS article
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EID of the result in the Scopus database
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