Utilization of high-accuracy FTICR-MS data in protein quantitation experiments

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F09%3A00010450" target="_blank" >RIV/61989592:15310/09:00010450 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/09:00338590 RIV/61989592:15110/09:00009505

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Utilization of high-accuracy FTICR-MS data in protein quantitation experiments

Original language description

Human acute T-lymphoblastic leukemia cell line (CEM) treated with cisplatin, and the stable isotope labeling by amino acids in cell culture (SILAC) strategy were used to present an improved method of data processing in high-accuracy mass spectrometry (MS). By using peptide mass fingerprinting with low mass tolerance, we were able to utilize far more data retained in MS scans which would normally be missed by a standard processing method. This new way of data interpretation results in an improvement of the relevance of quantitation experiments and enabled us to search and quantify different types of posttranslational modifications. Furthermore, we used this technique to distinguish among different protein isoforms, commonly returned by Mascot search engine.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/LC07017" target="_blank" >LC07017: Oncoproteomics Centre</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Mass Spectrometry

ISSN

1076-5174

e-ISSN

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Volume of the periodical

44

Issue of the periodical within the volume

11

Country of publishing house

DE - GERMANY

Number of pages

6

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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