MEKC Determination of Nilutamide in Human Serum Using Sweeping in High Salt Sample Matrix
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F11%3A33116273" target="_blank" >RIV/61989592:15310/11:33116273 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s10337-011-2019-1" target="_blank" >http://dx.doi.org/10.1007/s10337-011-2019-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10337-011-2019-1" target="_blank" >10.1007/s10337-011-2019-1</a>
Alternative languages
Result language
angličtina
Original language name
MEKC Determination of Nilutamide in Human Serum Using Sweeping in High Salt Sample Matrix
Original language description
The determination of the antiandrogen drug nilutamide in human serum by MEKC using flutamide as an internal standard is described. Several parameters influencing the separation, such as the running electrolyte composition concerning the micelle concentration and pH, are discussed. MEKC separation was achieved within 7 min using 50 mM sodium borate pH 9.0 with the addition of 50 mM sodium dodecylsulfate at +20 kV. The proposed method was applied to determination of nilutamide in spiked human serum samples after protein precipitation with acetonitrile. The increasing of sensitivity for determination of nilutamide in serum was done by sweeping in a high salt concentration sample matrix when the injection of a larger volume of sample diluted in 150 mM NaClwas applied. The limit of detection after the preconcentration step for nilutamide was 26 mu g L(-1).
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
<a href="/en/project/ED2.1.00%2F03.0058" target="_blank" >ED2.1.00/03.0058: Regional Centre of Advanced Technologies and Materials</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Chromatographia
ISSN
0009-5893
e-ISSN
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Volume of the periodical
74
Issue of the periodical within the volume
1
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
5
Pages from-to
151-155
UT code for WoS article
000291745400019
EID of the result in the Scopus database
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