Analysis of the Developmental Roles of the Arabidopsis Gibberellin 20-Oxidases Demonstrates That GA20ox1, -2, and -3 Are the Dominant Paralogs

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F12%3A33142871" target="_blank" >RIV/61989592:15310/12:33142871 - isvavai.cz</a>

Result on the web

<a href="http://www.plantcell.org/content/24/3/941" target="_blank" >http://www.plantcell.org/content/24/3/941</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1105/tpc.111.095109" target="_blank" >10.1105/tpc.111.095109</a>

Result language

angličtina

Original language name

Analysis of the Developmental Roles of the Arabidopsis Gibberellin 20-Oxidases Demonstrates That GA20ox1, -2, and -3 Are the Dominant Paralogs

Original language description

Gibberellin (GA) biosynthesis is necessary for normal plant development, with later GA biosynthetic stages being governed by multigene families. Arabidopsis thaliana contains five GA 20-oxidase (GA20ox) genes, and past work has demonstrated the importance of GA20ox1 and -2 for growth and fertility. Here, we show through systematic mutant analysis that GA20ox1, -2, and -3 are the dominant paralogs; their absence results in severe dwarfism and almost complete loss of fertility. In vitro analysis revealedthat GA20ox4 has full GA20ox activity, but GA20ox5 catalyzes only the first two reactions of the sequence by which GA(12) is converted to GA(9). GA20ox3 functions almost entirely redundantly with GA20ox1 and -2 at most developmental stages, including thefloral transition, while GA20ox4 and -5 have very minor roles. These results are supported by analysis of the gene expression patterns in promoter:beta-glucuronidase reporter lines. We demonstrate that fertility is highly sensitive to GA

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

ED - Physiology

OECD FORD branch

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Project

<a href="/en/project/KAN200380801" target="_blank" >KAN200380801: Immunonanotechnologies for hormone diagnostic</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Cell

ISSN

1040-4651

e-ISSN

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Volume of the periodical

24

Issue of the periodical within the volume

3

Country of publishing house

US - UNITED STATES

Number of pages

21

Pages from-to

941-960

UT code for WoS article

000303763000010

EID of the result in the Scopus database

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