Preconceptional paternal glycidamide exposure affects embryonic gene expression: Single embryo gene expression study following in vitro fertilization

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F86652036%3A_____%2F11%3A00371924" target="_blank" >RIV/86652036:_____/11:00371924 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.reprotox.2011.09.005" target="_blank" >http://dx.doi.org/10.1016/j.reprotox.2011.09.005</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.reprotox.2011.09.005" target="_blank" >10.1016/j.reprotox.2011.09.005</a>

Result language
angličtina
Original language name
Preconceptional paternal glycidamide exposure affects embryonic gene expression: Single embryo gene expression study following in vitro fertilization
Original language description
Recognition of early determinants of disease onset has sparked an interest in paternally transmitted factors and their impact on the developing embryo. Acrylamide (AA),a widely distributed xenobiotic compound,is converted to its active metabolite glycidamide (GA) by the CYP2E1 enzyme.Based on its capacity to induce dominant lethal mutations,we hypothesized that paternal GA exposure would have a negative impact on embryonic genome activation,via GA-DNA and protamine adducts persisting in the fertilizingsperm. Using a combination of in vitro fertilization (IVF) techniques and RT-qPCR single embryo gene expression (SEGE), we studied the expression of key DNA repair genes and genes important for embryo development, at the 1-, 2-, 4- and 8-cell stage of the developing mouse embryo. Compared to controls paternal GA-exposure gave rise to an altered pattern of embryonic gene expression, with an initial reduced expression at early stages followed by increased expression at the 8-cell stage.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
<a href="/en/project/IAA500520809" target="_blank" >IAA500520809: Mechanisms of embryonic stem cells early differentiation</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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