Gene expression during minor genome activation in preimplantation bovine development

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F09%3A00328317" target="_blank" >RIV/67985904:_____/09:00328317 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Gene expression during minor genome activation in preimplantation bovine development

Original language description

The main goal of this study was to identify mRNA transcripts, whose content increases during bovine minor embryonic genome activation. We compared the gene expression profile of the bovine 4-cell stage embryo and MII oocyte using the technique of suppression subtractive hybridization. Differentially expressed amplicons were subcloned and 60 of them were sequenced. The resulting DNA sequences were compared with GenBank databases using Blast search. The expression of five differentially expressed genes with an apparent function in cell cycle progression, chromatin remodeling, and splicing or translation initiation was further characterized by a real-time RT-PCR. Splicing factor, arginine/serine-rich 3 (SRFS3) represents the first described gene with an important function in pre-implantation development, which is also expressed during bovine minor genome activation and it is alpha-amanitin sensitive during this period. All described genes can play an important role in the pre-implantation

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GI - Farm animal breeding and farm animal pedigree breeding

OECD FORD branch

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Project

<a href="/en/project/GA523%2F06%2F1226" target="_blank" >GA523/06/1226: Embryonics of pre-implantation mammalian development</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Theriogenelogy

ISSN

0093-691X

e-ISSN

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Volume of the periodical

72

Issue of the periodical within the volume

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Country of publishing house

US - UNITED STATES

Number of pages

12

Pages from-to

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UT code for WoS article

000268901700019

EID of the result in the Scopus database

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