MDF is a conserved splicing factor and modulates cell division and stress response in Arabidopsis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F22%3A00563830" target="_blank" >RIV/61388971:_____/22:00563830 - isvavai.cz</a>
Result on the web
<a href="https://www.life-science-alliance.org/content/6/1/e202201507" target="_blank" >https://www.life-science-alliance.org/content/6/1/e202201507</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.26508/lsa.202201507" target="_blank" >10.26508/lsa.202201507</a>
Alternative languages
Result language
angličtina
Original language name
MDF is a conserved splicing factor and modulates cell division and stress response in Arabidopsis
Original language description
The coordination of cell division with stress response is essential for maintaining genome stability in plant meristems. Proteins involved in pre-mRNA splicing are important for these processes in animal and human cells. Based on its homology to the splicing factor SART1, which is implicated in the control of cell division and genome stability in human cells, we analyzed if MDF has similar functions in plants. We found that MDF associates with U4/U6.U5 tri-snRNP proteins and is essential for correct splicing of 2,037 transcripts. Loss of MDF function leads to cell division defects and cell death in meristems and was associated with up-regulation of stress-induced genes and down-regulation of mitotic regulators. In addition, the mdf-1 mutant is hypersensitive to DNA damage treatment supporting its role in coordinating stress response with cell division. Our analysis of a dephosphomutant of MDF suggested how its protein activity might be controlled. Our work uncovers the conserved function of a plant splicing factor and provides novel insight into the interplay of pre-mRNA processing and genome stability in plants.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Life Science Alliance
ISSN
2575-1077
e-ISSN
2575-1077
Volume of the periodical
6
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
21
Pages from-to
e202201507
UT code for WoS article
000876974400001
EID of the result in the Scopus database
2-s2.0-85140283164