Access of inhibitory neurosteroids to the NMDA receptor

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F12%3A33143002" target="_blank" >RIV/61989592:15310/12:33143002 - isvavai.cz</a>
Alternative codes found
RIV/67985823:_____/12:00379112 RIV/61388963:_____/12:00379112
Result on the web
<a href="http://onlinelibrary.wiley.com/doi/10.1111/j.1476-5381.2011.01816.x/abstract;jsessionid=AC688D0796D6A68D8D0E67DA50B1D2A7.d04t03" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1111/j.1476-5381.2011.01816.x/abstract;jsessionid=AC688D0796D6A68D8D0E67DA50B1D2A7.d04t03</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/j.1476-5381.2011.01816.x" target="_blank" >10.1111/j.1476-5381.2011.01816.x</a>

Result language
angličtina
Original language name
Access of inhibitory neurosteroids to the NMDA receptor
Original language description
BACKGROUND AND PURPOSE NMDA receptors are glutamatergic ionotropic receptors involved in excitatory neurotransmission, synaptic plasticity and excitotoxic cell death. Many allosteric modulators can influence the activity of these receptors positively ornegatively, with behavioural consequences. 20-Oxo-5 beta-pregnan-3a-yl sulphate (pregnanolone sulphate; PA-6) is an endogenous neurosteroid that inhibits NMDA receptors and is neuroprotective. We tested the hypothesis that the interaction of PA-6 with the plasma membrane is critical for its inhibitory effect at NMDA receptors. EXPERIMENTAL APPROACH Electrophysiological recordings and live microscopy were performed on heterologous HEK293 cells expressing GluN1/GluN2B receptors and cultured rat hippocampal neurons. KEY RESULTS Our experiments showed that the kinetics of the steroid inhibition were slow and not typical of drug-receptor interaction in an aqueous solution. In addition, the recovery from steroid inhibition was accelerated by
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
ED - Physiology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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