Crystals of Na /K -ATPase with bound cisplatin
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F14%3A33150766" target="_blank" >RIV/61989592:15310/14:33150766 - isvavai.cz</a>
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S000629521400519X" target="_blank" >http://www.sciencedirect.com/science/article/pii/S000629521400519X</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bcp.2014.08.029" target="_blank" >10.1016/j.bcp.2014.08.029</a>
Alternative languages
Result language
angličtina
Original language name
Crystals of Na /K -ATPase with bound cisplatin
Original language description
Cisplatin is the most widely used chemotherapeutics for cancer treatment, however, its administration is connected to inevitable adverse effects. Previous studies suggested that cisplatin is able to inhibit Na+/K+-ATPase (NKA), the enzyme responsible formaintaining electrochemical potential and sodium gradient across the plasma membrane. Here we report a crystallographic analysis of cisplatin bound to NKA in the ouabain bound E2P form. Despite a moderate resolution (7.4 angstrom and 7.9 angstrom), theanomalous scattering from platinum and a model representation from a recently published structure enabled localization of seven cisplatin binding sites by anomalous difference Fourier maps. Comparison with NKA structures in the E1P conformation suggestedtwo possible inhibitory mechanisms for cisplatin. Binding to Met151 can block the N-terminal pathway for transported cations, while binding to Met171 can hinder the interaction of cytoplasmic domains during the catalytic cycle.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biochemical Pharmacology
ISSN
0006-2952
e-ISSN
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Volume of the periodical
92
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
5
Pages from-to
494-498
UT code for WoS article
000345955400010
EID of the result in the Scopus database
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