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Disparate HDV ribozyme crystal structures represent intermediates on a rugged free-energy landscape

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F14%3A33152334" target="_blank" >RIV/61989592:15310/14:33152334 - isvavai.cz</a>

  • Alternative codes found

    RIV/68081707:_____/14:00431756 RIV/00216224:14740/14:00076675

  • Result on the web

    <a href="http://dx.doi.org/10.1261/rna.044982.114" target="_blank" >http://dx.doi.org/10.1261/rna.044982.114</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1261/rna.044982.114" target="_blank" >10.1261/rna.044982.114</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Disparate HDV ribozyme crystal structures represent intermediates on a rugged free-energy landscape

  • Original language description

    The hepatitis delta virus (HDV) ribozyme is a member of the class of small, self-cleaving catalytic RNAs found in a wide range of genomes from HDV to human. Both pre- and post-catalysis (precursor and product) crystal structures of the cis-acting genomicHDV ribozyme have been determined. These structures, together with extensive solution probing, have suggested that a significant conformational change accompanies catalysis. A recent crystal structure of a trans-acting precursor, obtained at low pH andby molecular replacement from the previous product conformation, conforms to the product, raising the possibility that it represents an activated conformer past the conformational change. Here, using fluorescence resonance energy transfer (FRET), we discovered that cleavage of this ribozyme at physiological pH is accompanied by a structural lengthening in magnitude comparable to previous trans-acting HDV ribozymes. Conformational heterogeneity observed by FRET in solution appears to have

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CF - Physical chemistry and theoretical chemistry

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2014

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    RNA-A Publication of the RNA Society

  • ISSN

    1355-8382

  • e-ISSN

  • Volume of the periodical

    20

  • Issue of the periodical within the volume

    7

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    17

  • Pages from-to

    1112-1128

  • UT code for WoS article

    000338271200016

  • EID of the result in the Scopus database