Fluorescent in situ Hybridization shows DIPLOSPOROUS located on one of the NOR chromosomes in apomictic dandelions in the absence of a large hemizygous chromosomal region

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F14%3A33155515" target="_blank" >RIV/61989592:15310/14:33155515 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1139/gen-2014-0143" target="_blank" >http://dx.doi.org/10.1139/gen-2014-0143</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1139/gen-2014-0143" target="_blank" >10.1139/gen-2014-0143</a>

Result language

angličtina

Original language name

Fluorescent in situ Hybridization shows DIPLOSPOROUS located on one of the NOR chromosomes in apomictic dandelions in the absence of a large hemizygous chromosomal region

Original language description

Apomixis in dandelions is encoded by two unlinked dominant loci and a third, yet undefined genetic factor: diplosporous omission of meiosis (DIPLOSPOROUS; DIP), parthenogenetic embryo development (PARTHENOGENESIS; PAR), and autonomous endosperm formation, respectively. In this study, we determined the chromosomal position of the DIP locus in Taraxacum by using fluorescent in situ hybridization (FISH) with bacterial artificial chromosomes (BACs) that genetically map within 1.2-0.2 cM of DIP. The BACs showed dispersed fluorescent signals, except for S4-BAC 83 that displayed strong unique signals as well. Under stringent blocking of repeats by C0t-DNA fragments, only a few fluorescent foci restricted to defined chromosome regions remained, including one on the nucleolus organizer region (NOR) chromosomes that contain the 45SrDNAs. FISH with S4-BAC 83 alone and optimal blocking showed discrete foci in the middle of the long arm of one of the NOR chromosomes only in triploid and tetraploid

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EF - Botany

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Genome (print)

ISSN

0831-2796

e-ISSN

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Volume of the periodical

57

Issue of the periodical within the volume

11-12

Country of publishing house

CA - CANADA

Number of pages

12

Pages from-to

609-620

UT code for WoS article

000353303900005

EID of the result in the Scopus database

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