Bulk derivatization and cation exchange restricted access media-based trap-and-elute liquid chromatography-mass spectrometry method for determination of trace estrogens in serum

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F15%3A33156897" target="_blank" >RIV/61989592:15310/15:33156897 - isvavai.cz</a>
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S0003267014013907" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0003267014013907</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.aca.2014.11.032" target="_blank" >10.1016/j.aca.2014.11.032</a>

Result language
angličtina
Original language name
Bulk derivatization and cation exchange restricted access media-based trap-and-elute liquid chromatography-mass spectrometry method for determination of trace estrogens in serum
Original language description
Estrone (E1), estradiols (alpha/beta-E2), and estriol (E3) are four major metabolically active estrogens exerting strong biological activities at very low circulating concentrations. This paper reports a sensitive and efficient method with automated, on-line clean-up and detection to determine trace estrogens in a small volume of serum samples using liquid chromatography-electrospray ionization-tandem mass spectrometry directly, without off-line liquid-liquid or solid-phase extraction pretreatments. Serum aliquots (charcoal stripped fetal bovine serum, 100mL) were spiked with four estrogen standards and their corresponding isotope-labeled internal standards, then bulk derivatized with 2-fluoro-1-methylpyridium p-toluenesulfonate (2-FMP) to establish the calibration curves and perform method validation. Calibration was established in the concentration ranges of 5-1000 pg/mL, and demonstrated good linearity of R2 from 0.9944 to 0.9997 for the four derivatized estrogens. The lower detecti
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Project
<a href="/en/project/EE2.4.31.0130" target="_blank" >EE2.4.31.0130: Development and bolstering of cooperation among academic and commercial institutions with focuse on chemical and pharmaceutical research</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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