Terahertz Raman Optical Activity Reveals Emergent Supramolecular Chirality in Mononucleotide G-quadruplexes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F25%3A73631306" target="_blank" >RIV/61989592:15310/25:73631306 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11320/25:10499295
Result on the web
<a href="https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/cmtd.202500070" target="_blank" >https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/cmtd.202500070</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/cmtd.202500070" target="_blank" >10.1002/cmtd.202500070</a>
Alternative languages
Result language
angličtina
Original language name
Terahertz Raman Optical Activity Reveals Emergent Supramolecular Chirality in Mononucleotide G-quadruplexes
Original language description
Raman optical activity (ROA) provides unique chiroptical insights into biomolecular structure but suffers from inherently weak signals. A substantial enhancement in the low-wavenumber/THz region (50–250 cm−1/1.5–7.5 THz) of ROA spectra upon the formation of mononucleotide G-quadruplexes (mG4) from guanosine-5′-monophosphate (5′rGMP) is reported. Using concentration-dependent Raman and ROA measurements, a nearly 100-fold increase in ROA intensity upon aggregation is identified, with spectral features highly sensitive to cation-mediated structural variations. These effects can be traced to long-range chiral interactions and distinct stacking arrangements within mG4. While strong terahertz Raman optical activity (THz-ROA) signals have previously been observed in globular proteins and α-helical polypeptides, the findings of this study represent the first evidence of this phenomenon in nucleotides, demonstrating that noncovalent self-assembly into supramolecular helices can give rise to intense low-frequency chiroptical responses. This establishes THz-ROA as a powerful background-free tool for studying supramolecular chirality and nucleotide self-assembly, with potential applications in characterizing biologically relevant G-quadruplex structures. © 2025 The Author(s). Chemistry - Methods published by Chemistry Europe and Wiley-VCH GmbH.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10306 - Optics (including laser optics and quantum optics)
Result continuities
Project
<a href="/en/project/GA25-15726S" target="_blank" >GA25-15726S: Exploration of new forms of Raman optical activity</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
CHEMISTRY-METHODS
ISSN
2628-9725
e-ISSN
2628-9725
Volume of the periodical
5
Issue of the periodical within the volume
11
Country of publishing house
DE - GERMANY
Number of pages
7
Pages from-to
"e202500070-1"-"e202500070-7"
UT code for WoS article
001518981400001
EID of the result in the Scopus database
2-s2.0-105009441747