Coligand-Dependent Cellular Effects and DNA/BSA Binding of Ruthenium(II) Tris(pyrazolylmethane) Complexes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F25%3A73633167" target="_blank" >RIV/61989592:15310/25:73633167 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15640/25:73633167
Result on the web
<a href="https://pubs.acs.org/doi/10.1021/acs.inorgchem.5c04198?src=getftr&utm_source=clarivate&getft_integrator=clarivate" target="_blank" >https://pubs.acs.org/doi/10.1021/acs.inorgchem.5c04198?src=getftr&utm_source=clarivate&getft_integrator=clarivate</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.inorgchem.5c04198" target="_blank" >10.1021/acs.inorgchem.5c04198</a>
Alternative languages
Result language
angličtina
Original language name
Coligand-Dependent Cellular Effects and DNA/BSA Binding of Ruthenium(II) Tris(pyrazolylmethane) Complexes
Original language description
Monocationic [RuCl(kappa 3-tpm)(L)(PPh3)]Cl (L = PPh3, 1; NCMe, 2; 1,3,5-triaza-7-phosphaadamantane (PTA), 3; phosphinoferrocene, 4; 3-methyl-pyrazole, 5; NH2(CH2)2OH, 6; NH2(CH2)2(4-C6H4OH) (tyramine), 7; cyclohexylamine, 8; NH2CH2CH2NH2, 9; tpm = tris-pyrazolylmethane) and bis-cationic ruthenium complexes [RuCl(kappa 3-tpm)(PPh3)(LL')][NO3]2 (LL ' = ethylenediamine, 10; 1,10-phenanthroline, 11; 2-picolylamine, 12; N-phenyl-1-(2-pyridinyl)methanimine, 13) and [RuCl(kappa 3-tpm)(PPh3)(NCMe)2][NO3]2 (14) were evaluated for their anticancer potential. Complexes 4-9 and 13-14 are novel and were obtained in 72-98% yields from thermal exchange reactions of 1. They were characterized by IR and multinuclear NMR spectroscopy, and the solid-state structures of 4, 5, 6, 7, and 14 were determined by single-crystal X-ray diffraction. Complexes 3-8 and 10-14 were further examined for solubility and stability in aqueous media, and octanol/water partition coefficients. The complexes were assessed for their in vitro cytotoxicity on a panel of six cancer and two normal cell lines. Complex 1 and the ruthenium-ferrocenyl conjugate 4 revealed significant-to-moderate activity against the cancer cells, with IC50 values ranging from 1.8 to 25.2 mu M. Mechanistic studies in A2780 cells included time-dependent cytotoxicity, intracellular ruthenium uptake, cell cycle analysis, autophagy induction, production of ROS (reactive oxygen species), and mitochondrial membrane potential measurements. Moreover, a detailed study was conducted to evaluate DNA and bovine serum albumin (BSA) binding capacity. Overall, the results revealed distinct potential mechanisms of action driven by ligand diversity, specifically mitochondrial uncoupling for 1 and 4, and apoptosis- and necrosis-induced cell death for 13 and 14.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10402 - Inorganic and nuclear chemistry
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
INORGANIC CHEMISTRY
ISSN
0020-1669
e-ISSN
1520-510X
Volume of the periodical
64
Issue of the periodical within the volume
50
Country of publishing house
US - UNITED STATES
Number of pages
19
Pages from-to
"24615–24633"
UT code for WoS article
001632715900001
EID of the result in the Scopus database
2-s2.0-105025198363