The plant and callus formation from bipolar structures excised from the surface of Nicotiana tabacum L. cultured leaves

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F00%3A21101003" target="_blank" >RIV/62156489:43210/00:21101003 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
The plant and callus formation from bipolar structures excised from the surface of Nicotiana tabacum L. cultured leaves
Original language description
The culture (more than 3 weeks) of explanted tobacco leaves on a MS medium containing 0.8 mg.l-1 2,4-D, 0.5 mg.l-1 BAP and 13.2 mg.l-1 aspartic acid produced callus, globular and bipolar structures. These structures resembled somatic embryos and showed numerous abnormalities and fasciations, but they had a developed root apex. The microscopic analysis of bipolar structures indicated the presence of meristematic tissues with dividing cells in the root apex. In the callus the tracheary elements were observed. Formation of secondary globules was frequent on different parts of primary globules and/or bipolar structures. The highest frequency of the bipolar structure formation was observed when the air was regularly exchanged by venting the cultures of
Czech name
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Czech description
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Project
<a href="/en/project/VS96082" target="_blank" >VS96082: Hormonal regulation of plant growth and development</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2000
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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