Capillary Electrophoretic Studies of Acid-Base Properties of Sanguinarine and Chelerythrine Alkaloids.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F04%3A00000829" target="_blank" >RIV/62156489:43210/04:00000829 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Capillary Electrophoretic Studies of Acid-Base Properties of Sanguinarine and Chelerythrine Alkaloids.

Original language description

Capillary zone electrophoresis with UV detection was used for determination of dissociation constants of alkaloids sanguinarine and chelerythrine. Despite the limited solubility of the uncharged forms of the alkaloids resulting in insufficient analyticalsignal at higher pH the reliable dissociation constants were obtained when acidified samples containing low amount of the alkaloid were injected into the capillary. The precipitation of the alkaloid in the capillary induced by injecting sample of low pHinto the background electrolyte of higher pH does not affect the mobility of the alkaloid if its concentration injected exceeds the solubility only to a small extent. Dissociation constants (pKR+) of sanguinarine and chelerythrine calculated to 8.3 +- 0.1 and 9.2 +- 0.1, respectively, are relevant to Good buffers of ionic strength of 30 mM.

Czech name

CE stanovení disociačních konstant

Czech description

Capillary zone electrophoresis with UV detection was used for determination of dissociation constants of alkaloids sanguinarine and chelerythrine. Despite the limited solubility of the uncharged forms of the alkaloids resulting in insufficient analyticalsignal at higher pH the reliable dissociation constants were obtained when acidified samples containing low amount of the alkaloid were injected into the capillary. The precipitation of the alkaloid in the capillary induced by injecting sample of low pHinto the background electrolyte of higher pH does not affect the mobility of the alkaloid if its concentration injected exceeds the solubility only to a small extent. Dissociation constants (pKR+) of sanguinarine and chelerythrine calculated to 8.3 +- 0.1 and 9.2 +- 0.1, respectively, are relevant to Good buffers of ionic strength of 30 mM.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

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Volume of the periodical

1040

Issue of the periodical within the volume

1

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

5

Pages from-to

141-145

UT code for WoS article

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EID of the result in the Scopus database

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