The use of HPLC for determination of expression product of microbial tyrosine decarboxylase

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F07%3A00114129" target="_blank" >RIV/62156489:43210/07:00114129 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

čeština

Original language name

Využití HPLC ke stanovení produktu exprese genu pro mikrobiální tyrosindekarboxylasu

Original language description

The aim of this study was verification of the presence or absence of specific DNA sequences coding for tyrosine decarboxylase in starter cultures using for fermented sausages production and confirmation of the tyrosine decarboxylase gene expression usingrapid HPLC analysis of the expression product - tyramin. Genomic DNA from single microorganisms using as starter cultures for fermented sausages was extracted using fenol extraction procedure. This DNA was used as a template for PCR identification. Theset of oligonucleotide primers based on tyrosine decarboxylase gene sequence was used in PCR. Two strains with high tyrosine decarboxylase activity were detected (Lactobacillus curvatus and Lactobacillus sake). The tyrosine decarboxylase gene expressionof these two strains was analyzed using optimalized rapid HPLC method which confirmed the presence of high concentrations of tyramine. These results show suitability of described PCR and HPLC method for the skríning of starter cultures fo

Czech name

Využití HPLC ke stanovení produktu exprese genu pro mikrobiální tyrosindekarboxylasu

Czech description

The aim of this study was verification of the presence or absence of specific DNA sequences coding for tyrosine decarboxylase in starter cultures using for fermented sausages production and confirmation of the tyrosine decarboxylase gene expression usingrapid HPLC analysis of the expression product - tyramin. Genomic DNA from single microorganisms using as starter cultures for fermented sausages was extracted using fenol extraction procedure. This DNA was used as a template for PCR identification. Theset of oligonucleotide primers based on tyrosine decarboxylase gene sequence was used in PCR. Two strains with high tyrosine decarboxylase activity were detected (Lactobacillus curvatus and Lactobacillus sake). The tyrosine decarboxylase gene expressionof these two strains was analyzed using optimalized rapid HPLC method which confirmed the presence of high concentrations of tyramine. These results show suitability of described PCR and HPLC method for the skríning of starter cultures fo

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

GM - Food industry

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Chemické listy

ISSN

0009-2770

e-ISSN

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Volume of the periodical

101

Issue of the periodical within the volume

11

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

4

Pages from-to

907-910

UT code for WoS article

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EID of the result in the Scopus database

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