Novel detection of c131y mutation using allele specific PCR (AS ? PCR)

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F08%3A9P000445" target="_blank" >RIV/62156489:43210/08:9P000445 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—

Result language
angličtina
Original language name
Novel detection of c131y mutation using allele specific PCR (AS ? PCR)
Original language description
In the present study we deal with detecting SNP in myostatin (MSTN) gene using molecular genomic methods. MSTN is a negative regulator of muscle growth. Nine different mutations were found in that gene. These are responsible for double muscling in cattleand the others species. Mutation called G938A was detected in Gasconne cattle breed and it is causing embarrassment during birth. The aim of breeders is to suppress appearance of phenotype trait of double muscling in Gasconne sires, mainly. By mentionedreasons, two different methods were designed. First was based on allele specific PCR. This method obtained positive results with comparing of results from sequencing fragment. Novel method was verified using another one. The primers were designed usingon line software Primer 3 (v 0.4.0). The samples were analysed in ABI PRISM 310 machine with using DNA Sequencing Analysis Software (ver. 5.1).
Czech name
Nový způsob detekce mutace C313Y pomocí alelle specific PCR (AS-PCR)
Czech description
Pomocí AS-PCR byla detekována mutace C313Y u plemene gasconne. Mutace je odpovědná za dvojite osvaleni u tohoto plemene. Důvěryhodnost detekce pomoci nové metody byla ověřena sekvenovanim daného úseku.

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
—

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2008
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)