An effect of cadmium and lead ions on Escherichia coli with the cloned gene for metallothionein (MT-3) revealed by electrochemistry

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F14%3A00225094" target="_blank" >RIV/62156489:43210/14:00225094 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14740/14:00076328 RIV/00216305:26620/14:PU111625

Result on the web

<a href="http://www.sciencedirect.com/science/article/pii/S0013468614012730" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0013468614012730</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.electacta.2014.06.091" target="_blank" >10.1016/j.electacta.2014.06.091</a>

Result language

angličtina

Original language name

An effect of cadmium and lead ions on Escherichia coli with the cloned gene for metallothionein (MT-3) revealed by electrochemistry

Original language description

This study was focused on the application of electrochemical methods for studying of bacterial strains Escherichia coli and Escherichia coli expressing human metallothionein gene (MT-3) before and after the application of cadmium and/or lead ions in fourconcentrations (25, 50, 75 and 150 micrometre). Bacterial strains Escherichia coli and Escherichia coli expressing human metallothionein gene (MT-3) were used like model organisms for studying of metals influence to metallothionein expression. Metallothionein was isolated using fast protein liquid chromatography and quantified by electrochemical methods. The occurrence of metallothionein in E.coli was confirmed by gel electrophoresis by the presence of the bands at 15 (MT dimer) and 22 kDa (MT trimer).The changes in electrochemical records due to the interactions of metallothioneins (MT-3 and MT-2A) with cadmium and lead ions showed decline of Cat2 signal of MT with the increasing interaction time because of metal ions binding to cyst

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CG - Electrochemistry

OECD FORD branch

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Project

<a href="/en/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: Central european institute of technology</a><br>

Continuities

O - Projekt operacniho programu

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electrochimica Acta

ISSN

0013-4686

e-ISSN

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Volume of the periodical

140

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

9

Pages from-to

11-19

UT code for WoS article

342528600003

EID of the result in the Scopus database

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