Primer efficiency determination for flavanone 3-hydroxylase gene in wheat (Triticum aestivum L.)

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F14%3A00229015" target="_blank" >RIV/62156489:43210/14:00229015 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—

Result language
angličtina
Original language name
Primer efficiency determination for flavanone 3-hydroxylase gene in wheat (Triticum aestivum L.)
Original language description
Anthocyanins are water-soluble pigments that may be red, purple, or blue. They belong to a parent class of molecules called flavonoids. Anthocyanins occur in all tissues of higher plants, including leaves, stems, roots, blossoms, and fruits. Flavanon 3-hydroxylase (F3H) is one of the key enzymes of the flavanoid biosynthesis pathway. F3H converts flavanones to dihydroflavonols. The main aim was to study the F3H gene in wheat (Triticum aestivum L.). In our experiment 5 genotypes with nonstandard colouredcaryopses were used (2 genotypes with blue aleurone, 2 genotypes with purple pericarp and 1 genotype with white caryopses- without anthocyanins). Total RNA was isolated by phenol-chloroform method and complementary DNA was obtained by reverse transcription. Specific primers for each of the F3H genes (F3H_A, F3H_B and F3H_D) that were found in the National Center for Biotechnology Information (NCBI) database were designed. The first results of quantitative PCR (qPCR) contained two or mor
Czech name
—
Czech description
—

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
MendelNet 2014 - Proceedings of International PhD Students Conference
ISBN
978-80-7509-174-1
ISSN
—
e-ISSN
—
Number of pages
5
Pages from-to
469-473
Publisher name
Mendel University in Brno
Place of publication
Brno, Czech Republic
Event location
Brno, Czech Republic
Event date
Nov 19, 2014
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
—

Similar results(10)