Effect of PC-3 prostate cancer cell line supernatant on apoptosis in macrophages
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F15%3A43906883" target="_blank" >RIV/62156489:43210/15:43906883 - isvavai.cz</a>
Result on the web
<a href="https://mnet.mendelu.cz/mendelnet2015/mnet_2015_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2015/mnet_2015_full.pdf</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Effect of PC-3 prostate cancer cell line supernatant on apoptosis in macrophages
Original language description
Particular types of cell death, for example apoptosis, play important role in metastatic processes. Apoptosis is programmed cell death, which is characterised by specific morphological changes. In this study we aimed on topic of affecting of supernatantfrom prostate cancer cell line PC-3 on a healthy cells of immune system, macrophages concretely. Peripheral blood monocytes were cultivated for 7 days to macrophages. Macrophages were stimulated for 24 hours by lipopolysaccharide (LPS) and cultivated with supernatant for another 24 hours. Preparations were prepared and analysed by light microscopy. Macrophages with normal morphology show the largest rate in our experiment. In samples macrophages + supernatant there was an increase mainly in late stage of apoptosis. A lot of observed cells showed feature of rupture and spillage of cell contents into the microenvironment. In samples macrophages + supernatant + LPS there is a noticeable decline in the incidence of normal morphology compare
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
FD - Oncology and haematology
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
MendelNet 2015: Proceedings of International PhD Students Conference
ISBN
978-80-7509-363-9
ISSN
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e-ISSN
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Number of pages
5
Pages from-to
470-474
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Nov 11, 2015
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000366466100089