Effect of prostate cancer cell line supernatant on functional polarization in macrophages

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F18%3A43913911" target="_blank" >RIV/62156489:43210/18:43913911 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14110/18:00104058

Result on the web

<a href="https://doi.org/10.4149/BLL_2018_095" target="_blank" >https://doi.org/10.4149/BLL_2018_095</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4149/BLL_2018_095" target="_blank" >10.4149/BLL_2018_095</a>

Result language

angličtina

Original language name

Effect of prostate cancer cell line supernatant on functional polarization in macrophages

Original language description

OBJECTIVES: We aimed on effect of supernatant derived from prostate cancer cell line PC-3 on M1/M2 functional polarization in macrophages. BACKGROUND: Cytokines play an important role in carcinogenesis. Most of them are produced by macrophages. Macrophages are divided into groups M1 or M2. Classical phenotype macrophages M1 support pro-inflammatory effects and produce pro-infl ammatory cytokines, such as interleukin 6 (IL-6), interleukin 12 (IL-12), tumor necrosis factor α (TNF-α). Macrophages exhibiting a phenotype M2 secrete anti-infl ammatory cytokines, e. g. interleukin 10 (IL-10), transforming growth factor β (TGF-β). METHODS: Peripheral blood monocytes were cultivated for 7 days and during this time went through a differentiation into macrophages. Macrophages were stimulated for 24 hours by lipopolysaccharide (LPS) as a positive control and cultivated with supernatant for another 24 hours. RESULTS: Macrophages cultivated without LPS and without supernatant were used as negative control. Relative expression of IL-6, IL-10, IL-12 and TNF-α was measured by Quantitative real-time PCR. Expression of pro-infl ammatory cytokines was lower in macrophages with supernatant compared to positive control. CONCLUSION: Expression of pro-infl ammatory cytokines was lower in macrophages with supernatant (MΦ+sup) compared to positive control (MΦ+LPS). Effect of the supernatant on expression of IL-6, IL-10, IL-12 and TNF-α was not confi rmed (Tab. 1, Fig. 5, Ref. 15).

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30204 - Oncology

Project

—

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Bratislavské lekárske listy (Bratislava Medical Journal)

ISSN

0006-9248

e-ISSN

—

Volume of the periodical

119

Issue of the periodical within the volume

8

Country of publishing house

SK - SLOVAKIA

Number of pages

6

Pages from-to

516-521

UT code for WoS article

000444369100011

EID of the result in the Scopus database

2-s2.0-85052808089