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ELISA-like Analysis of Cisplatinated DNA Using Magnetic Separation

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F15%3A43907734" target="_blank" >RIV/62156489:43210/15:43907734 - isvavai.cz</a>

  • Result on the web

    <a href="http://www.intechopen.com/journals/nanobiomedicine/elisa-like-analysis-of-cisplatinated-dna-using-magnetic-separation" target="_blank" >http://www.intechopen.com/journals/nanobiomedicine/elisa-like-analysis-of-cisplatinated-dna-using-magnetic-separation</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.5772/61699" target="_blank" >10.5772/61699</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    ELISA-like Analysis of Cisplatinated DNA Using Magnetic Separation

  • Original language description

    Cisplatin belongs to the most widely used cytostatic drugs. The determination of the presence of the DNA-cisplatin adducts may not only signal the guanine-rich regions but also monitor the interaction reaction between DNA and the drug in terms of speed of interaction.In this work, the combined advantages of magnetic particles-based isolation/purification with fluorescent properties of quantum dots (QDs) and antibodies targeted on specific recognition of DNA-cisplatin adducts are demonstrated. The formation of a complex between magnetic particles with surface modified by anti-dsDNA antibody, cisplatin-modified DNA and QDs labelled anti-cisplatin-modified DNA antibody was suggested and optimized.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CE - Biochemistry

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GAP102%2F11%2F1068" target="_blank" >GAP102/11/1068: Nano-Electro-Bio-Tools for Biochemical and Molecularly-Biological Studies of Eukaryotic Cells (NanoBioTECell)</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2015

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Nanobiomedicine

  • ISSN

    1849-5435

  • e-ISSN

  • Volume of the periodical

    2

  • Issue of the periodical within the volume

    10

  • Country of publishing house

    HR - CROATIA

  • Number of pages

    8

  • Pages from-to

    "nestrankovano"

  • UT code for WoS article

  • EID of the result in the Scopus database