Stability of Reference Genes Estimated by Real-Time PCR in Porcine Liver
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F16%3A43910212" target="_blank" >RIV/62156489:43210/16:43910212 - isvavai.cz</a>
Result on the web
<a href="https://mnet.mendelu.cz/mendelnet2016/mnet_2016_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2016/mnet_2016_full.pdf</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Stability of Reference Genes Estimated by Real-Time PCR in Porcine Liver
Original language description
It is essential for the method real-time PCR to control variables by validation of normalization of the data. The aim of this study was to develop a set of reference genes for relative quantification of mRNA expression in the porcine liver. The mRNA stability of expression was studied for five genes: GAPDH, HPRT1, PPIA, TOP2B, TBP1, where for each gene the Ct value characterized the level of expression in liver. With the help of geNorm range of stability of analysed genes was (from the most stable to the least): PPIA, TBP1, TOP2B, GAPDH, HPRT1.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
GI - Farm animal breeding and farm animal pedigree breeding
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
MendelNet 2016: Proceedings of International PhD Students Conference
ISBN
978-80-7509-443-8
ISSN
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e-ISSN
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Number of pages
4
Pages from-to
815-818
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Nov 9, 2016
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
000392968500146