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Stability of Reference Genes Estimated by Real-Time PCR in Porcine Liver

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F16%3A43910212" target="_blank" >RIV/62156489:43210/16:43910212 - isvavai.cz</a>

  • Result on the web

    <a href="https://mnet.mendelu.cz/mendelnet2016/mnet_2016_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2016/mnet_2016_full.pdf</a>

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Stability of Reference Genes Estimated by Real-Time PCR in Porcine Liver

  • Original language description

    It is essential for the method real-time PCR to control variables by validation of normalization of the data. The aim of this study was to develop a set of reference genes for relative quantification of mRNA expression in the porcine liver. The mRNA stability of expression was studied for five genes: GAPDH, HPRT1, PPIA, TOP2B, TBP1, where for each gene the Ct value characterized the level of expression in liver. With the help of geNorm range of stability of analysed genes was (from the most stable to the least): PPIA, TBP1, TOP2B, GAPDH, HPRT1.

  • Czech name

  • Czech description

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    GI - Farm animal breeding and farm animal pedigree breeding

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2016

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    MendelNet 2016: Proceedings of International PhD Students Conference

  • ISBN

    978-80-7509-443-8

  • ISSN

  • e-ISSN

  • Number of pages

    4

  • Pages from-to

    815-818

  • Publisher name

    Mendelova univerzita v Brně

  • Place of publication

    Brno

  • Event location

    Brno

  • Event date

    Nov 9, 2016

  • Type of event by nationality

    EUR - Evropská akce

  • UT code for WoS article

    000392968500146