Complex genome rearrangements in an Arabidopsis T-DNA line

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F17%3A43912586" target="_blank" >RIV/62156489:43210/17:43912586 - isvavai.cz</a>
Result on the web
<a href="https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Complex genome rearrangements in an Arabidopsis T-DNA line
Original language description
In recent years, an increasing number of studies demonstrated that many Arabidopsis T-DNA lines might carry massive genome changes as a direct consequence of the transfer DNA (T-DNA) integration event. In this work we report an analysis of a segregating T-DNA mutant line (SAIL_218_G01), which contains such genomic rearrangements. The initial PCR analyses did not yield any homozygous plants, suggesting putative gametophyte lethality. However, this result is likely caused by chromosomal translocations, which were clearly identified in several heterozygous plants by a pollen viability assay. Surprisingly, we identified plants that carried the T-DNA and still produced 100% viable pollen. For reciprocal balanced translocations, these plants should be then homozygous for the T-DNA insertion. Yet, the offspring of some of these plants still segregated the T-DNA, suggesting more complex genome rearrangements in the studied Arabidopsis mutant line.
Czech name
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Czech description
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Project
<a href="/en/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
MendelNet 2017: Proceedings of International PhD Students Conference
ISBN
978-80-7509-529-9
ISSN
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e-ISSN
neuvedeno
Number of pages
5
Pages from-to
608-612
Publisher name
Mendelova univerzita v Brně
Place of publication
Brno
Event location
Brno
Event date
Nov 8, 2017
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
000440194500108

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