Detection of ROS Generated by UV-C Irradiation of CdS Quantum Dots and their Effect on Damage to Chromosomal and Plasmid DNA

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F18%3A43913547" target="_blank" >RIV/62156489:43210/18:43913547 - isvavai.cz</a>

Alternative codes found

RIV/00216305:26620/18:PU127820

Result on the web

<a href="https://doi.org/10.1002/elan.201700648" target="_blank" >https://doi.org/10.1002/elan.201700648</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elan.201700648" target="_blank" >10.1002/elan.201700648</a>

Result language

angličtina

Original language name

Detection of ROS Generated by UV-C Irradiation of CdS Quantum Dots and their Effect on Damage to Chromosomal and Plasmid DNA

Original language description

Different sensitivity of various types of DNA to damage and mutation requires a study of effects of chemical and physical factors on integrity of individual DNA structures. Oxidation stress induced by photoactivated nanoparticles including quantum dots is considered to be the main mechanism of their potential risks. In this work, spectrophotometric indicators of reactive oxygen species (ROS) 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) and -diphenyl--picrylhydrazyl (DPPH), electrochemical DNA-based biosensors with the thiol-capped CdS quantum dots (CdS QDs) immobilized over the chromosomal (ct DNA) and plasmid (pUC19) DNA biorecognition layers and agarose gel electrophoresis were used (i) to detect ROS generated at the UV-C irradiation (=254nm) of CdS QDs and (ii) to assess and evaluate their effect towards the chromosomal and plasmid dsDNA structure. Voltammetric and impedimetric measurements revealed a deep degradation of DNA attached to the glassy carbon electrode surface with a stepwise decrease of the portion of survived pUC19 and ct DNA after 60s to 2400s irradiation time up to 47% and to 32%, resp., comparing to the portion in the absence of CdS QDs reaching 58% and 40%, resp. Agarose gel electrophoresis supported the damage to plasmid and chromosomal DNA demonstrating double strand breaks. A decrease in the stability of DNA types towards degradation by ROS in order pUC19&gt;calf thymus&gt;salmon sperm dsDNA has been found.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10406 - Analytical chemistry

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electroanalysis

ISSN

1040-0397

e-ISSN

—

Volume of the periodical

30

Issue of the periodical within the volume

4

Country of publishing house

DE - GERMANY

Number of pages

7

Pages from-to

698-704

UT code for WoS article

000429708900016

EID of the result in the Scopus database

2-s2.0-85045411959